Premium
Elastomeric polyacrylamide gels for high‐resolution electrophoresis of proteins
Author(s) -
Aizawa Katsunori
Publication year - 2000
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/1099-1581(200008/12)11:8/12<481::aid-pat44>3.0.co;2-#
Subject(s) - elastomer , materials science , polyacrylamide , resolution (logic) , polyacrylamide gel electrophoresis , electrophoresis , polymer science , high resolution , chromatography , composite material , polymer chemistry , biology , chemistry , biochemistry , artificial intelligence , computer science , archaeology , enzyme , history
The inclusion of dextran in polyacrylamide crosslinked with N,N′‐(1,2‐dihydroxyethylene)bisacrylamide (DHEBA) produced elastomeric gels. Enhancement of the resolving power of the gel electrophoresis for sodium dodecyl sulfate‐protein complexes (molecular mass from 14,400 to 97,400) was observed by using the elastomeric gels. Their effective pore sizes were smaller, compared with conventional polyacrylamide gels crosslinked with DHEBA at the same total monomer (T) and crosslinker (C) concentrations. The molecular sieving network of the elastomeric gel may be formed by crosslinked polyacrylamide and entangled dextran molecules. Copyright © 2000 John Wiley & Sons, Ltd.