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Biological activity of chitosan–sugar hybrids: specific interaction with lectin
Author(s) -
Li Xuebing,
Tushima Yohsuke,
Morimoto Minoru,
Saimoto Hiroyuki,
Okamoto Yoshiharu,
Minami Saburo,
Shigemasa Yoshihiro
Publication year - 2000
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/1099-1581(200004)11:4<176::aid-pat964>3.0.co;2-z
Subject(s) - chitosan , lectin , concanavalin a , agglutinin , residue (chemistry) , pseudomonas aeruginosa , fucose , biochemistry , chemistry , biology , bacteria , glycoprotein , in vitro , genetics
The specific interactions between lectins and chitosan–sugar hybrids, the synthesized chitosan derivatives linking carbohydrate residue to the amino group of chitosan, were investigated. The specific bindings of chitosan‐ L ‐fucose (Fuc) hybrid with Ulex europaeus agglutinin I (UEA I, a lectin specific to L ‐Fuc), and chitosan‐ N ‐acetyl‐ D ‐glucosamine ( D ‐GlcNAc) hybrid with Concanavalin A (Con A, a lectin specific to D ‐glucose, D ‐mannose and D ‐GlcNAc), were confirmed by a surface plasmon resonance technique. The microscopic observation of Pseudomonas aeruginosa , which was preincubated with the fluorescein isothiocyanate‐labeled chitosan‐ L ‐Fuc hybrid, showed bacteria aggregation. The aggregation was thought to be resulted from the specific interaction of the L ‐Fuc residue of the hybrid with PA‐II lectin on the surface of P. aeruginosa . The chitosan‐ L ‐Fuc hybrid inhibited P. aeruginosa growth more effectively in comparison with the other hybrids or unmodified chitosan. The enhancement of antimicrobial activity of chitosan‐ L ‐Fuc hybrid could be attributed to the specific binding between PA‐II lectin of P. aeruginosa and L ‐Fuc residue of the L ‐Fuc hybrid. Copyright © 2000 John Wiley & Sons, Ltd.