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Inhibitory effects of Sudanese medicinal plant extracts on hepatitis C virus (HCV) protease
Author(s) -
Hussein Ghazi,
Miyashiro Hirotsugu,
Nakamura Norio,
Hattori Masao,
Kakiuchi Nobuko,
Shimotohno Kunitada
Publication year - 2000
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/1099-1573(200011)14:7<510::aid-ptr646>3.0.co;2-b
Subject(s) - traditional medicine , protease , medicinal plants , hepatitis c virus , biology , pharmacognosy , in vitro , virus , medicine , biological activity , virology , enzyme , biochemistry
One hundred fifty‐two methanol and water extracts of different parts of 71 plants commonly used in Sudanese traditional medicine were screened for their inhibitory effects on hepatitis C virus (HCV) protease (PR) using in vitro assay methods. Thirty‐four extracts showed significant inhibitory activity (≥60% inhibition at 100 µg/mL). Of these, eight extracts, methanol extracts of Acacia nilotica, Boswellia carterii, Embelia schimperi, Quercus infectoria, Trachyspermum ammi and water extracts of Piper cubeba, Q. infectoria and Syzygium aromaticum , were the most active (≥90% inhibition at 100 µg/mL). From the E. schimperi extract, two benzoquinones, embelin (I) and 5‐ O ‐methylembelin (II), were isolated and found as potent HCV‐PR inhibitors with IC 50 values of 21 and 46 µ M , respectively. Inhibitory activities of derivatives of I against HCV‐PR as well as their effects on other serine proteases were also investigated.Copyright © 2000 John Wiley & Sons, Ltd.