Premium
Application of flow injection—chemiluminescence to the study of radical scavenging activity in plants
Author(s) -
Choi Hong Yeob,
Jhun Eun Ja,
Lim Beong Ou,
Chung Ill Min,
Kyung Suk Hun,
Park Dong Ki
Publication year - 2000
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/1099-1573(200006)14:4<250::aid-ptr587>3.0.co;2-j
Subject(s) - caffeic acid , chemistry , gallic acid , butylated hydroxytoluene , catechin , chemiluminescence , antioxidant , hydroxyl radical , hydrogen peroxide , linoleic acid , organic chemistry , medicinal chemistry , polyphenol , fatty acid
Chemiluminescence (CL) was observed during the oxidation of luminol (2 mg/L). mediated by 0.06% hydrogen peroxide (H 2 O 2 ) and cytochrome c (10 mg/L). CL intensity was decreased by the presence of radical scavengers and the reduction was linearly proportional to the concentration and ability of scavengers; butylated hydroxytoluene (BHT), caffeic acid and gallic acid. The order of effectiveness as radical scavengers was gallic acid > caffeic acid > BHT, which shows that the number of hydroxyl groups (OH) in the B‐ring of flavonoids plays a key role in a good radical scavenging activity. Of eight catechins obtained from green tea extracts, (−)‐catechin was the least effective and (−)‐epigallocatechin gallate (EGCg) showed the strongest activity. This result indicates that the stereoscopic structure between the C‐3 group and the B ring of flavonoids as well as substituents at the C‐3 position make a contribution to radical scavenging activity. Of the tested Chinese herbal ingredients, five species of ingredients represented more than 90% of the radical scavenging activity. Copyright © 2000 John Wiley & Sons, Ltd.