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Capillary gel electrophoretic separation of superoxide dismutases in leaf extracts of Triticum aestivum L.
Author(s) -
Chen ZuLiang,
Zhu Yongguan
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/1099-1565(200011/12)11:6<362::aid-pca542>3.0.co;2-u
Subject(s) - chemistry , capillary electrophoresis , chromatography , detection limit , superoxide dismutase , electrophoresis , capillary action , sodium , calibration curve , molecular mass , relative standard deviation , analytical chemistry (journal) , enzyme , biochemistry , materials science , organic chemistry , composite material
Capillary gel electrophoretic (CGE) separation of metal superoxide dismutase (SOD; EC 1.15.1.1) types is described. A mixture of standard SOD types was fully denatured into SDS–SOD complexes in the presence of excess sodium dodecylsulphate (SDS). The SDS–SOD complexes were separated using an uncoated fused silica capillary filled with a polymer running buffer and detected spectrophotometrically at 200 nm. Three types of SOD were separated, namely, Cu/ZnSOD (32 kDa), FeSOD (38 kDa) and MnSOD (40 kDa). Calibration curves were linear in the concentration range of 0.1–5.0 mg/mL with a detection limit in the order of 0.05 mg/mL. The reproducibility of the migration time was high with a relative standard deviation of less than 1.50%. The proposed method was demonstrated to be applicable to the separation of SOD types in leaf extracts of wheat. Copyright © 2000 John Wiley & Sons, Ltd.