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Rapid analysis of apolar low molecular weight constituents in wood using high pressure liquid chromatography with evaporative light scattering detection
Author(s) -
Claassen Frank W.,
Haar Cees van de,
van Beek Teris A.,
Dorado José,
MartínezIñigo MaríaJose,
SierraAlvarez Reyes
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/1099-1565(200007/08)11:4<251::aid-pca522>3.0.co;2-p
Subject(s) - chemistry , chromatography , elution , size exclusion chromatography , dichloromethane , fractionation , acetone , softwood , acetic acid , resin acid , extraction (chemistry) , acetonitrile , dissolution , sample preparation , methanol , solvent , organic chemistry , botany , biology , enzyme
A new high pressure liquid chromatographic method with evaporative light scattering detection was developed for the qualitative and quantitative analysis of apolar, low molecular weight constituents in wood. The wood extractives were obtained by means of a 6 h Soxhlet extraction with acetone. The extract was quantitatively analysed on an end‐capped C18 column eluted with a gradient of acetonitrile:water:acetic acid. The crude sample was directly injected following dissolution in acetonitrile:methanol:dichloromethane (60:8:40) and filtration through a 0.45 µm membrane filter. No other time consuming fractionation or derivatisation steps were required prior to the analysis. The main apolar, low molecular weight constituents of pinewood are free fatty acids, resin acids, sterols and triglycerides. With this method, individual free fatty acids, sterols and triglycerides are well separated. Resin acids, with the exception of dehydroabietic acid, are not separated and co‐elute. The method does not require any sample clean‐up steps except filtration and it is compatible with other softwood and hardwood wood species. Copyright © 2000 John Wiley & Sons, Ltd.