Premium
Evaluation of liquid chromatography–atmospheric pressure chemical ionisation–mass spectrometry for the identification and quantification of desulphoglucosinolates
Author(s) -
Griffiths D. Wynne,
Bain Henry,
Deighton Nigel,
Botting Nigel P.,
Robertson Avril A. B.
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/1099-1565(200007/08)11:4<216::aid-pca526>3.0.co;2-2
Subject(s) - chemistry , chromatography , mass spectrometry , atmospheric pressure , analytical chemistry (journal) , liquid chromatography–mass spectrometry , chemical ionization , sample preparation , high performance liquid chromatography , calibration curve , atmospheric pressure chemical ionization , detection limit , ionization , organic chemistry , ion , oceanography , geology
The use of liquid chromatography–atmospheric pressure chemical ionisation–mass spectrometry has been investigated as a potential method for both the identification and quantification of desulphoglucosinolates. The results indicate that this methodology is particularly useful as a rapid technique for the confirmation of the identity of desulphoglucosinolates and has, in full scan mode, limits of detection at least an order of magnitude lower than is normally achieved by conventional high pressure liquid chromatography with UV detection. It has been demonstrated that, using a per‐deuterated desulphoglucosinolate as an internal standard, quantification could be achieved, but for accurate analysis at low concentrations it would be necessary to produce independent calibration curves for each desulphoglucosinolate present in the sample. Copyright © 2000 John Wiley & Sons, Ltd.