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Non‐invasive tumour blood perfusion measurement by 2 H magnetic resonance
Author(s) -
Bentzen L.,
Horsman M. R.,
Daugaard P.,
Maxwell R. J.
Publication year - 2000
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/1099-1492(200012)13:8<429::aid-nbm663>3.0.co;2-k
Subject(s) - perfusion , nuclear magnetic resonance , laser doppler velocimetry , nuclear medicine , magnetic resonance imaging , nuclear magnetic resonance spectroscopy , chemistry , blood flow , biomedical engineering , medicine , radiology , physics
Deuterium uptake into foot‐implanted C3H murine mammary carcinomas was measured non‐invasively by 2 H NMR spectroscopy at 46 MHz after i.v. injection. The arterial input function (AIF) was estimated from 2 H NMR measurements with a second radiofrequency coil externally located over the heart. Tumour and heart data were acquired over the same time period by means of a switch automatically activated every 1.6–3.2 s. Although the AIF data were, in general, partly contaminated by signals from adjacent tissue, a mathematical fitting procedure involving simultaneous fitting of the AIF and the tumour kinetics gave robust results for tumour blood perfusion (TBP): up to four repeat TBP measurements were made in 14 out of 20 untreated animals and TBP could be measured before and after treatment in 14 out of 15 animals. The ability of this technique to measure changes in blood perfusion was assessed using hydralazine, which decreased TBP from 91 to 29 ml 100 g −1 min −1 and this was comparable to a 70% reduction in relative TBP measured by laser Doppler flowmetry. Copyright © 2001 John Wiley & Sons, Ltd. Abbreviations used: AIF arterial input functionAUC area under the curveHdz hydralazineTBP tumour blood perfusion