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Magnetic susceptibility shift selected imaging (MESSI) and localized 1 H 2 O spectroscopy in living plant tissues
Author(s) -
Zhong Kai,
Li Xin,
ShacharHill Yair,
Picart Francis,
Wishnia Arnold,
Springer Charles S.
Publication year - 2000
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/1099-1492(200011)13:7<392::aid-nbm659>3.0.co;2-o
Subject(s) - xylem , chemistry , extracellular , nuclear magnetic resonance , intracellular , nuclear magnetic resonance spectroscopy , stele , phloem , biophysics , diamagnetism , paramagnetism , spectroscopy , in vivo , biochemistry , botany , biology , magnetic field , stereochemistry , physics , quantum mechanics , microbiology and biotechnology
Maize root segments permeated with aqueous solutions of the paramagnetic agents GdDTPA 2− or DyDTPA‐BMA display two well‐resolved NMR peaks corresponding to the signals from intracellular and extracellular 1 H 2 O, which arise from well‐understood bulk magnetic susceptibility effects. This allows each component to be studied separately. Images obtained at each frequency with MESSI editing, and single‐ and multiple‐voxel (‘spectroscopic imaging’) localized spectra, clearly indicate that the agents permeate into the interstitial spaces, and into the longitudinal (xylem/phloem) channels in the stele (core) of the root, confirming earlier assessments. We believe these are the first images of a multicellular tissue acquired in vivo exclusively from the intracellular water proton resonance. This method can be further exploited to study water transport in similar systems. Copyright © 2000 John Wiley & Sons, Ltd. Abbreviations used: BMS bulk magnetic susceptibilityEDTA ethylenediamine tetraacetateDTPA diethylenetriamine pentaacetateDTPA‐BMA 3− diethylenetriamine pentaacetate bis‐methylamide.