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Quantitation by 1 H‐NMR of dolichol, cholesterol and choline‐containing lipids in extracts of normal and phathological thyroid tissue
Author(s) -
Yoshioka Yoshichika,
Sasaki Jun,
Yamamoto Masahide,
Saitoh Kazuyoshi,
Nakaya Shigeyuki,
Kubokawa Manabu
Publication year - 2000
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/1099-1492(200011)13:7<377::aid-nbm658>3.0.co;2-e
Subject(s) - dolichol , cholesterol , medicine , chemistry , thyroid , endocrinology , adenoma , biochemistry , enzyme , biology , biosynthesis
Proton magnetic resonance spectroscopy at 1.9 T was used to quantify dolichols, cholesterols, choline‐containing phospholipids and double bonds in unsaturated acyl chains in lipid extracts of four types of thyroid tissue [normal ( n = 27), papillary cancer ( n = 15), adenoma ( n = 13) and Basedow disease ( n = 6)]. In normal thyroid the mean concentrations of dolichol, cholesterol and phospholipids were 1.2, 3.6 and 2.1 µmol/g wet weight, respectively. The concentrations of these lipids exhibited positive mutual correlations and positive correlations with patient age. The increase in dolichol in elderly human thyroid may be due to the accumulation of lysosomes and may help to compensate for the decrease in the activity of lysosomal enzymes and in thyroid hormone production and release. Dolichol concentrations were significantly lower in papillary cancer (0.4 µmol/g) and Basedow disease (0.3 µmol/g) compared to normal thyroid ( p < 0.01 and p < 0.05, respectively), while cholesterol was enhanced only in cancer tissue (10.7 µmol/g). Benign adenoma exhibited normal levels of both dolichol and cholesterol. These results suggest that the synthesis and accumulation of isoprenoids are normal in adenoma but not in cancer. Copyright © 2000 John Wiley & Sons, Ltd. Abbreviations used: PC phosphatidylcholineSPH sphingomyelinTSH thyroid stimulating hormone