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Double‐resonance J ‐edited 1 H‐NMR detection of [6‐ 13 C]‐ D ‐2‐deoxyglucose uptake in glioma cells
Author(s) -
Knijn A.,
Casieri C.,
Carpinelli G.,
Testa C.,
Podo F.,
De Luca F.
Publication year - 2000
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/1099-1492(200005)13:3<124::aid-nbm618>3.0.co;2-c
Subject(s) - deoxyglucose , nuclear magnetic resonance , pulse sequence , glioma , chemistry , methylene , glucose uptake , solvent exposure , in vitro , carbon 13 nmr , solvent , biology , physics , biochemistry , cancer research , insulin , organic chemistry , endocrinology
The C6 methylene protons were selectively detected in 1 H‐NMR spectra of intact glioma cells incubated with [6‐ 13 C]‐ D ‐2‐deoxyglucose ([6‐ 13 C]‐2dG), a 13 C‐enriched glucose analog that is suitable for monitoring glucose utilization in brain tumors. Spectral editing via 1 H– 13 C scalar coupling was performed with twin spin‐echo double resonance (T‐SEDOR), a pulse sequence which combines chemical specificity and high sensitivity, requires no solvent pre‐saturation, and can easily be adapted to imaging protocols. This work demonstrates the suitability of the pulse sequence for monitoring [6‐ 13 C]‐2dG uptake in living cells in vitro , in spite of line‐broadening and the occurrence of other strong signals in the spectral region of interest (3.5–4.4 ppm). Copyright © 2000 John Wiley & Sons, Ltd.