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New feature of angiotensin‐converting enzyme: carbohydrate‐recognizing domain
Author(s) -
Kost Olga A.,
Bovin Nicolai V.,
Chemodanova Elena E.,
Nasonov Vitaly V.,
Orth Tatiana A.
Publication year - 2000
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/1099-1352(200011/12)13:6<360::aid-jmr508>3.0.co;2-k
Subject(s) - chemistry , oligosaccharide , glycoprotein , biochemistry , monosaccharide , enzyme , dimer , carbohydrate , angiotensin converting enzyme , renin–angiotensin system , biology , organic chemistry , endocrinology , blood pressure
Self carbohydrate‐mediated dimerization of glycoprotein angiotensin‐converting enzyme (ACE) was demonstrated. The dimerization was studied in the reverse micelle experimental system as a model of biomembrane situation. Asialo‐ACE or agalacto‐ACE was able to form a dimer, whereas deglycosylated ACE and sequentially desialylated and degalactosylated ACE failed to dimerize. ACE–ACE interaction was competitively inhibited by Neu5Ac‐ or Gal‐terminated saccharides. The results have allowed us to propose the existence of carbohydrate‐recognizing domain (CRD) on ACE molecule. The structural requirements of this CRD were estimated based on the ability of saccharides to inhibit ACE dimerization. The most effective monosaccharides with equal inhibition potencies were shown to be galactose (as GalβOMe) and N ‐acetylneuraminic acid (as Neu5AcαOMe). Among oligosaccharides, the most effective ones were found to be 3′SiaLac and, especially, the whole pool of ACE oligosaccharide chains and biantennae complex oligosaccharide chains of other glycoproteins. Bovine and human ACEs were shown to be similar in terms of recognition of carbohydrates. Copyright © 2000 John Wiley & Sons, Ltd.Abbreviations used : ACE angiotensin‐converting enzymeAOT aerosol OT, bis‐(2‐ethylhexyl)‐sulfosuccinate, sodium saltCRD carbohydrate‐recognizing domainFA‐Phe‐Gly‐Gly N‐(3‐[2‐furyl]acryloyl)‐ L ‐phenylalanyl‐glycyl‐glycineKDN ketodeoxynonulosonic acidPAA poly[ N ‐(2‐hydroxyethyl)acrylamide]SiaLe xNeu5Acα2‐3Galβ1‐4(Fucα1‐3)GlcNAcβ.