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Quantitative and sequence‐specific analysis of DNA–ligand interaction by means of fluorescent intercalator probes
Author(s) -
Kirschstein Omar,
Sip Miroslav,
Kittler Leonhard
Publication year - 2000
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/1099-1352(200005/06)13:3<157::aid-jmr498>3.0.co;2-y
Subject(s) - fluorescence , intercalation (chemistry) , dna , sequence (biology) , ligand (biochemistry) , chemistry , computational biology , biophysics , sequence analysis , biochemistry , biology , receptor , organic chemistry , optics , physics
A novel method of analysis of double‐stranded DNA–ligand interaction is presented. The interaction is monitored by the fluorescence of a DNA bis‐intercalator oxazole homodimer YoYo‐3. The fluorescence intensity or its decay time reflects the modification of the DNA double helix. The DNA sequence is scanned by hybridization with short oligomers having consecutively overlapping complementary sequences to analyse the sequence specificity of binding. In our experiments we used as ligands the minor groove binders netropsin, SN6999 (both with AT‐preference), the GC‐specific ligand chromomycin A3 as well as the derivative SN6113 (non‐specific interaction), which displace the bis‐intercalator YoYo‐3 or influence the duplex structure in such away that the fluorescence intensity and lifetime decrease in comparison to a ligand‐free screening. The changes of fluorescence emission clearly define the binding motif and indicate minor groove interactions with a reduced DNA binding site. Titration of the ligand quantitatively characterizes its binding by determining the dependence of the binding constant on the oligonucleotide sequence. Copyright © 2000 John Wiley & Sons, Ltd.