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Preparation of photoreactive 12‐[(4‐azidosalicyl)amino] dodecanoic acid acylated derivatives of gangliosides radioioiodinated to high specific radioactivity
Author(s) -
Pacuszka Tadeusz,
Panasiewicz Mirosława
Publication year - 2000
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/1099-1344(200011)43:13<1255::aid-jlcr414>3.0.co;2-s
Subject(s) - chemistry , residue (chemistry) , chloramine t , ganglioside , chromatography , high performance liquid chromatography , amino acid , derivative (finance) , biochemistry , organic chemistry , financial economics , economics
We describe the preparation of 125 I labeled 12[(4‐azidosalicyl)amino]dodecanoic acid (ASD) acylated derivatives of G M3 , G D3 , G M1 and SPG gangliosides. Gangliosides isolated from natural sources were deacylated, reacylated with ASD and radioiodinated with 125 I and chloramine T as an oxidant. During purification by HPLC each radioiodinated ganglioside derivative emerged from the column as two peaks differing in the substitution of 4‐azidosalicylic acid residue. Radioiodinated gangliosides‐ASD were of over 95% purity and about 2200 Ci/mmole specific activity. They were used for photolabeling of erythrocyte membrane proteins. Copyright © 2000 John Wiley & Sons, Ltd.