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Studies on urinary metabolites of perlolyrine by stable isotope tracer method
Author(s) -
Tang Ganghua,
Jiang Guohui,
Wang Shihchen
Publication year - 2000
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/1099-1344(200007)43:8<781::aid-jlcr362>3.0.co;2-c
Subject(s) - chemistry , metabolite , chromatography , hydrolysis , hydroxylation , methanol , ether , sulfuric acid , derivatization , mass spectrometry , alcohol , aqueous solution , organic chemistry , biochemistry , enzyme
After oral administration of perlolyrine and deuterated perlolyrine, the rat urines were collected, hydrolyzed with glucuronidase, basified with NaHCO 3 –Na 2 CO 3 , extracted with ethyl ether–iso‐propyl alcohol. The organic phases (neutral and basic fractions) were concentrated for conversion to TMS derivatives. The aqueous phase were acidified with sulfuric acid, taken to dryness under a nitrogen stream, extracted with methanol (water soluble acidic fractions) and concentrated for conversion to TMS derivatives. The TMS derivatives were determined by gas chromatography‐mass spectrometry (GC‐MS). Perlolyrine and one metabolite were found from the neutral and basic fractions, and two different metabolites were found from the water soluble acidic fractions. It was proposed that the major possible metabolic pathways of perlolyrine were the hydroxylation of perlolyrine and the oxidation of its hydroxylmethyl group. Copyright © 2000 John Wiley & Sons, Ltd.