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Separation and determination of strychnine and brucine in Strychnos nux‐vomica L. and its preparation by capillary zone electrophoresis
Author(s) -
Chen Wansheng,
Liu Lili,
Li Xiang,
Li Jiewei,
Ji Songgang,
Zhang Guoqing,
Chai Yifeng
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200012)14:8<541::aid-bmc49>3.0.co;2-r
Subject(s) - brucine , strychnine , chemistry , strychnos , chromatography , capillary electrophoresis , acetic acid , ammonium acetate , alkaloid , high performance liquid chromatography , stereochemistry , organic chemistry , biochemistry
A capillary zone electrophoresis method was developed for the separation and determination of strychnine and brucine in Strychnos nux‐vomica L . and its preparation. The factors that could affect the separation were studied, such as the types and concentrations of electrolytes, pH, ionic strength and organic modifier. The optimum running buffer was 20 mmol/L of ammonium acetate containing 0.2 mol/L of glacial acetic acid (pH 3.64). The applied voltage was 25 kV and the wavelength of the UV detector was set at 214 nm. The established method with dopamine hydrochloride as internal standard was linear in the range of 5–100 µg/mL for both strychnine and brucine. The recovery was 102.96% for strychnine and 98.56% for brucine. The extracts of Strychnos nux‐vomica and its preparation could be directly injected for analysis. Copyright © 2000 John Wiley & Sons, Ltd.