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Alkaline treatment of the cellulose fiber affecting membrane column behaviour for high‐performance immunoaffinity chromatography
Author(s) -
Zhou Dongmei,
Zou Hanfa,
Wang Hailin,
Ni Jianyi,
Zhang Qiang,
Zhang Yukui
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200012)14:8<511::aid-bmc16>3.0.co;2-b
Subject(s) - membrane , chemistry , chromatography , adsorption , cellulose , affinity chromatography , hollow fiber membrane , porosity , fiber , biochemistry , organic chemistry , enzyme
The original cellulose fibers and those treated by alkaline solution were both used to prepare the acrylic membranes. The two kinds of membranes were packed into the columns for high‐performance immunoaffinity chromatography by the immobilization of protein A on them. It was observed that the alkaline treatment of the cellulose fiber decreased the pressure resistance of the membrane to the mobile phases and greatly increased the accessible volume to the proteins, but affected the adsorption capacity of human IgG on the protein A membrane columns less. There is little difference between those two kinds of membranes on the adsorption capacities of HIgG, which means that the alkaline treatment of the cellulose fiber only significantly changes the void volume inter‐membrane, and the porosity and surface area of membrane less. Alkaline treatment of the cellulose fiber reduced the membrane‐column efficiency significantly. Some typical examples for the immunoaffinity analysis of IgG from human and dog plasma on the protein A membrane columns are illustrated. Copyright © 2000 John Wiley & Sons, Ltd. Abbreviations used: HREM high resolution electron microscopy.