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Chromatography of human prothrombin from Nitschmann fraction III on DEAE Sepharose Fast Flow using axial and radial flow column
Author(s) -
Sun Tao,
Chen Ge,
Liu Yiping,
Bu Fengrong,
Wen Meijuan
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200011)14:7<478::aid-bmc996>3.0.co;2-p
Subject(s) - chemistry , chromatography , column (typography) , volumetric flow rate , fraction (chemistry) , flow (mathematics) , analytical chemistry (journal) , packed bed , mechanics , geometry , mathematics , physics , connection (principal bundle)
An axial column (3 × 2.6 cm) and a radial flow column (3.5 × 5 cm) packed with DEAE Sepharose Fast Flow media was evaluated for the separation of human prothrombin from Nitschmann fraction III. Under radial flow conditions, a sample flow rate up to 14 mL/min (approximately 18 bed vols/h) was achieved. Breakthrough capacity was determined and both columns had almost the same breakthrough capacity per mL media, indicating that the sample loading was independent of radial column geometry. Copyright © 2000 John Wiley & Sons, Ltd.