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Enantiomeric determination of L ‐ and D ‐lactic acid in human cerebrospinal fluid by chiral ligand exchange high‐performance liquid chromatography
Author(s) -
Okubo Shigeo,
Mashige Fumiko,
Omori Mie,
Hashimoto Yoshiaki,
Nakahara Kazuhiko,
Kanazawa Hideko,
Matsushima Yoshikazu
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200011)14:7<474::aid-bmc995>3.0.co;2-3
Subject(s) - chemistry , chromatography , lactic acid , enantiomer , high performance liquid chromatography , extraction (chemistry) , ligand (biochemistry) , solid phase extraction , stereochemistry , receptor , bacteria , biochemistry , genetics , biology
Enantiomeric determination of L ‐ and D ‐lactate in human cerebrospinal fluid (CSF) was achieved by HPLC on a chiral stationary phase with UV detection. Samples were submitted to a solid‐phase extraction procedure using Oasis™ HLB Plus Extraction Cartridge and L ‐ and D ‐lactate in the extract were separated by Shodex ORpac CRX‐453 B column, a ligand exchange column for chiral separation, using a mobile phase containing copper (II) ion. L ‐ and D ‐lactate were determined in 25 min. Intra‐assay precision in CSF was 4.98% (mean 1.85 mmol/L) for L ‐lactate and 10.1% (mean 4.96 µmol/L) for D ‐lactate ( n = 5). Detection limits were between 1.0 ( L ‐lactate) and 1.5 ( D ‐lactate) pmol. The mean values ( n = 3) of analytical recovery for L ‐ and D ‐lactate were 95% and 107%, respectively. The mean ± SD of concentrations of L ‐ and D ‐lactate in CSF ( n = 20) were 1.52 ± 0.54 mmol/L and 10.98 ± 5.15 µmol/L, respectively. Copyright © 2000 John Wiley & Sons, Ltd.