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Hyphenated HPLC‐NMR and its applications in drug discovery
Author(s) -
Peng Sean X.
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200010)14:6<430::aid-bmc32>3.0.co;2-p
Subject(s) - chemistry , high performance liquid chromatography , chromatography , drug discovery , nuclear magnetic resonance spectroscopy , organic chemistry , biochemistry
Hyphenated HPLC‐NMR is a fast growing technology, allowing rapid and detailed structural characterization of unknown mixtures. The technical aspects of the technology are reviewed on the basis of system configuration, operation, solvent suppression, HPLC and NMR optimization, and detection. The combined use of HPLC‐NMR and HPLC‐MS is also described and discussed. Various applications of HPLC‐NMR and integrated HPLC‐NMR‐MS in drug discovery, especially in the separation and structure elucidation of drug impurities, reaction mixtures, degradation products, in vitro and in vivo metabolites, and combinatorial library samples, are illustrated. Copyright © 2000 John Wiley & Sons, Ltd. Abbreviations used: APCI atmospheric pressure chemical ionizationAPI atmospheric pressure ionizationCEC capillary electrochromatographyESI electrospray ionizationIT ion trapQ quadrupoleSFC supercritical fluid chromatographyTOF time‐of‐flight