Premium
Liquid chromatography–mass spectrometry in the study of the metabolism of drugs and other xenobiotics
Author(s) -
Oliveira E. J.,
Watson D. G.
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200010)14:6<351::aid-bmc28>3.0.co;2-2
Subject(s) - chemistry , chromatography , atmospheric pressure chemical ionization , mass spectrometry , xenobiotic , drug metabolism , electrospray ionization , liquid chromatography–mass spectrometry , solid phase extraction , sample preparation , ion suppression in liquid chromatography–mass spectrometry , chemical ionization , ion mobility spectrometry , extraction (chemistry) , metabolism , ionization , ion , organic chemistry , biochemistry , enzyme
The application of liquid chromatography–mass spectrometry (LC/MS) to the study of metabolism of drugs and other xenobiotics is reviewed. Original research papers covering the period from 1998 to early 2000 and concerning the use of LC/MS in the study of xenobiotic metabolism in humans and other mammalian species are reviewed. LC/MS interfaces, sample preparation steps, column types, mobile phases and additives, and the type of metabolites detected are summarized and discussed in an attempt to identify the current and future trends in the use of LC/MS for metabolism studies. Applications are listed according to the parent xenobiotic type and include substances used in therapeutics, drug candidates, compounds being evaluated in clinical trials, environmental pollutants, adulterants and naturally occurring substances. Copyright © 2000 John Wiley & Sons, Ltd. Abbreviations used: APCI atmospheric pressure chemical ionizationAPI atmospheric pressure ionizationCID collision‐induced dissociationCNL constant neural lossESI electrospray ionizationLC/MS liquid chromatography–mass spectrometryLLE liquid–liquid extractionSIM selective ion monitoringSPE solid phase extractionSRM selected reaction monitoringTOF time‐of‐flight.