Premium
Determination of morphine 3‐esters in rabbit plasma by high‐performance liquid chromatography with ultraviolet detection
Author(s) -
Otter Karin,
Mignat Christian,
Heber Dieter,
Ziegler Albrecht
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200008)14:5<327::aid-bmc991>3.0.co;2-g
Subject(s) - chemistry , chromatography , detection limit , morphine , high performance liquid chromatography , extraction (chemistry) , pharmacology , medicine
A sensitive high‐performance liquid chromatographic (HPLC) method for the quantitation of the morphine 3‐esters 1[3‐(2,2‐dimethylvaleroyl)‐morphine (A), 3‐(2‐phenylbenzoyl)‐morphine (B) and 3‐(2,2‐diphenylpropionyl)‐morphine (C)] in rabbit plasma is described. Sample preparation was based on reversed‐phase solid‐phase extraction. The compounds were separated on C 18 reversed‐phase analytical columns and then determined by ultraviolet detection. The recovery from plasma was 78.7 ± 7.4%, 69.1 ± 6.9% and 75 ± 7.2% (mean ± SD) for A, B, and C, respectively. The present method enabled the detection limit of 0.2, 0.2 and 0.1 ng and quantification limit of 20, 10 and 10 ng/ml for A, B and C, respectively. The developed method was used for determination of the plasmakinetics of these morphine 3‐esters in rabbits. Copyright © 2000 John Wiley & Sons, Ltd.