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High‐performance liquid chromatographic assay for the determination of novel triazole antifungal agents in tissue. Application to tissue distribution studies
Author(s) -
Khan Jehangir K.,
Montaseri Hashem,
Poglod Marzena,
Bu HaiZhi,
Daneshtalab Mohsen,
Micetich Ronald G.
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200008)14:5<321::aid-bmc990>3.0.co;2-0
Subject(s) - chemistry , chromatography , elution , acetonitrile , high performance liquid chromatography , acetone , matrix (chemical analysis) , protein precipitation , detection limit , homogenization (climate) , biochemistry , biodiversity , ecology , biology
A simple and rugged reversed‐phase high‐performance liquid chromatographic method with ultraviolet absorbance detection at 263 nm was developed and validated for the analysis of novel triazole antifungal agents SYN‐2869 and its derivatives in tissues. The method involved homogenization with 0.01 M phosphate buffer (pH 7.8) for lung, brain and spleen tissues. The liver and kidneys were homogenized with acetonitrile:acetone (1:1). The plasma proteins were precipitated with ice‐cold acetonitrile and supernatent was evaporated to dryness. The reconstituted samples were injected onto an HPLC system. SYN‐2869 was separated from the matrix components on a symmetry C 18 column using a aqueous mobile phase of acetonitrile and water with a flow rate of 1 mL/min. A step gradient of 40–80% acetonitrile eluted SYN‐2869 and the internal standard (SYN‐2506). The linear range was 0.5–10 µg/g ( r 2 > 0.99). The limit of quantitation was 0.5 µg/g. The inter‐day precision and accuracy for SYN 2869 standard concentration were from 2.6 to 7.4% and from −1.56 to +3.29%, respectively. The method was applied to tissue samples collected from single intravenous administration to mice to evaluate the distribution of these novel antifungal agents to different tissues. Copyright © 2000 John Wiley & Sons, Ltd.