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Determination of amobarbital and secobarbital in plasma samples using micellar liquid chromatography
Author(s) -
QuiñonesTorrelo C.,
MartínBiosca Y.,
Sagrado S.,
VillanuevaCamañas R. M.,
MedinaHernández M. J.
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200008)14:5<287::aid-bmc1>3.0.co;2-c
Subject(s) - secobarbital , chemistry , amobarbital , chromatography , detection limit , plasma , high performance liquid chromatography , anesthesia , pentobarbital , medicine , physics , quantum mechanics , neuroscience , epilepsy , biology
A new liquid chromatographic procedure for the determination of amobarbital and secobarbital in plasma samples is proposed. The method uses a Spherisorb octadecylsilane ODS‐2 C 18 analytical column, a guard column of similar characteristics and 0.04 M CTAB solution buffered at pH 7.5 containing 3% 1‐propanol as micellar mobile phase. The UV detection was carried out at 250 nm. Butabarbital was used as internal standard. Plasma samples preparation only required adequate dilution with the mobile phase before injection into the chromatographic system. The limits of detection were 0.2 and 0.4 mg/L for amobarbital and secobarbital, respectively. The proposed method allows the determination of amobarbital and secobarbital in plasma at therapeutic levels. Copyright © 2000 John Wiley & Sons, Ltd.