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Determination of Ibuprofen in human plasma by high‐performance liquid chromatography: validation and application in pharmacokinetic study
Author(s) -
Canaparo R.,
Muntoni E.,
Zara G. P.,
Della Pepa C.,
Berno E.,
Costa M.,
Eandi M.
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200006)14:4<219::aid-bmc969>3.0.co;2-z
Subject(s) - chemistry , chromatography , ibuprofen , pharmacokinetics , human plasma , plasma , high performance liquid chromatography , pharmacology , medicine , physics , quantum mechanics
A specific method for the simultaneous determination of S ‐(+)Ibuprofen and R ‐(−)Ibuprofen enantiomers in human plasma is described. Adopting a high‐performance liquid chromatographic (HPLC) system with spectrofluorometer detector, the compounds were extracted from plasma in alcohol medium and were separated on C18 column, using a solution of acetonitrile–water–acetic acid–triethylamine as mobile phase. The limit of quantitation was 0.1 µg/mL for both compounds. The method was validated by intra‐day assays at three concentration levels and was used in a kinetic study in healthy volunteers. During the study we carried out inter‐day assays to confirm the feasibility of the method. Copyright © 2000 John Wiley & Sons, Ltd.