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High‐performance liquid chromatography of monoamines on phenyl‐bound sorbents using organic free mobile phases
Author(s) -
Mezine Igor,
Chavez Mark,
Vatamaniuk Marko,
Teff Karen
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200005)14:3<205::aid-bmc976>3.0.co;2-n
Subject(s) - chemistry , chromatography , high performance liquid chromatography , monoamine neurotransmitter , phosphate buffered saline , elution , dopamine , tryptophan , phosphate , silica gel , resolution (logic) , serotonin , amino acid , organic chemistry , biochemistry , neuroscience , receptor , biology , artificial intelligence , computer science
Tryptophan and its metabolites, 5‐hydroxytryptophan, 5‐hydroxytryptamine, 5‐hydroxyindolacetic acid, as well as dopamine, homovanilic acid and 2,3‐dihydroxyphenylacetic acid, were separated on phenyl bound silica gel using isocratic elution with phosphate buffer. The method was successfully transferred to several other phenyl HPLC columns from different manufacturers simply by adjusting the pH of the buffer. The method has been validated by the determination of the level of monoamines in rat hypothalamus. Copyright © 2000 John Wiley & Sons, Ltd.