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Extraction and chromatographic separation methods in pharmacokinetic studies of Stobadine®—an indole‐related antioxidant and free‐radical scavenger
Author(s) -
S̆oltés L.,
Bezek S̆.,
Ujházy E.,
Bauer V.
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200005)14:3<188::aid-bmc974>3.0.co;2-f
Subject(s) - chemistry , bioanalysis , chromatography , pharmacokinetics , chromatographic separation , scavenger , extraction (chemistry) , drug metabolism , indole test , antioxidant , pharmacology , high performance liquid chromatography , metabolism , biochemistry , medicine
This overview provides comprehensive information on the most relevant results of Stobadine preclinical disposition studies. In order to investigate pharmacokinetic processes of the drug in rats, dogs and in human volunteers, several bioanalytical assays based on radiometric, spectrofluorometric, as well as chromatographic determination methods were developed and implemented. In small laboratory animals, the drug absorption, distribution, metabolism and elimination were investigated by administering 3 H‐labeled Stobadine. Spectrofluorometry was used alternatively for the determination of cold/unlabeled Stobadine in extracts of biomaterials sampled from larger animal species. The chromatographic separation methods proved, however, to be the most advantageous for determining details of the drug disposition and fate in the body. Copyright © 2000 John Wiley & Sons, Ltd.