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Determination of histamine, 1‐methylhistamine and N‐methylhistamine by capillary electrophoresis with micelles
Author(s) -
Nishiwaki Fumie,
Kuroda Koichi,
Inoue Yoshinori,
Endo Ginji
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200005)14:3<184::aid-bmc970>3.0.co;2-2
Subject(s) - histamine , chemistry , urine , chromatography , metabolite , capillary electrophoresis , medicine , biochemistry
Urinary histamine and N γ‐methylhistamine (1‐MH), a histamine metabolite, are highly correlated with histamine in plasma. Therefore, allergic reactions can be examined by determination of histamine and 1‐MH in urine. We separated histamine, 1‐MH and Nα‐methylhistamine (N‐MH) by capillary electrophoresis with UV detection at 210 nm, using borate buffer (pH 9) containing 100 m M SDS. The absolute detection limits were 200, 100 and 50 pg for histamine, 1‐MH and N‐MH, respectively. To purify histamine 1‐MH and N‐MH in urine, a silica cartridge was used. Recovery rates of histamine, 1‐MH and N‐MH in physiological saline were 90.0, 91.4 and 95.4%, respectively. We measured histamine and 1‐MH levels in urine from a normal female volunteer before and after a meal, and a male bronchial asthma patient. The results showed clearly that the concentrations of histamine and its metabolite rose after eating or asthma attack. N‐MH was not detected in the urine. Copyright © 2000 John Wiley & Sons, Ltd.