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Microdialysis sampling coupled to HPLC for transdermal delivery study of ondansetron hydrochloride in rats
Author(s) -
Ding Pingtian,
Xu Hui,
Wei Gang,
Zheng Junmin
Publication year - 2000
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/1099-0801(200005)14:3<141::aid-bmc937>3.0.co;2-z
Subject(s) - chemistry , transdermal , microdialysis , chromatography , high performance liquid chromatography , ondansetron , oleic acid , pharmacology , anesthesia , biochemistry , medicine , extracellular , nausea
The transdermal delivery of ondansetron hydrochloride (ON) solution in propylene glycol (PG) with a widely used penetration enhancer, oleic acid (OA), was studied in rats by a microdialysis sampling technique. Dialysate samples collected from the probe were directly injected into the HPLC system without any pre‐treatment and no interference occural in the blank sample. A good linearity between the standard concentrations and peak areas within the calibration range was achieved. In vivo recovery (32.52 ± 1.8%) of the probe was assessed with the retrodialysis method, which was used to calculate the ON concentration in the dermis. Oleic acid at the concentrations of 2% and 5% (w/v) increased the steady‐state delivery rate from 0.001 to 0.030 and 0.058 µg/h, respectively. OA proved to be an effective enhancer for transdermal delivery of ON in rats. Copyright © 2000 John Wiley & Sons, Ltd.