Premium
Synthesis and Characteristics of Biodegradable Pyridinium Amphiphiles Used for in vitro DNA Delivery
Author(s) -
Roosjen Astrid,
Šmisterová Jarmila,
Driessen Cecile,
Anders Joachim T.,
Wagenaar Anno,
Hoekstra Dick,
Hulst Ron,
Engberts Jan B. F. N.
Publication year - 2002
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/1099-0690(200204)2002:7<1271::aid-ejoc1271>3.0.co;2-g
Subject(s) - chemistry , pyridinium , amphiphile , in vitro , dna , combinatorial chemistry , nanotechnology , organic chemistry , biochemistry , copolymer , polymer , materials science
Pyridinium amphiphiles have found practical application for the delivery of DNA into eukaryotic cells. A general synthetic method starting from (iso)nicotinoyl chloride has been devised for the preparation of pyridinium amphiphiles based on (bio)degradable esters, allowing structural variation both in the hydrophobic part and in the headgroup area. By means of differential scanning calorimetry, transmission electron microscopy and UV measurements, some characteristics, including hydrolytic behaviour, have been determined. In vitro transfection ability and toxicity have been determined using the eukaryotic COS‐7 cell line. (© Wiley‐VCH Verlag GmbH, 69451 Weinheim, Germany, 2002)