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Noncovalently Bound Cofactors for Chemical Nucleases
Author(s) -
Roigk Anne,
Schneider HansJörg
Publication year - 2001
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/1099-0690(200101)2001:1<205::aid-ejoc205>3.0.co;2-f
Subject(s) - chemistry , cofactor , serine , histidine , imidazole , aspartic acid , catalysis , hydrolysis , dna , amino acid , phosphate , stereochemistry , protecting group , plasmid , biochemistry , combinatorial chemistry , organic chemistry , enzyme , alkyl
A large range of amino acids, including protected ones, were tested as cofactors in the Eu III ‐catalyzed hydrolysis of the biocidic bi(nitrophenyl)phosphate (BNPP) and of plasmid DNA. Serine, histidine, and aspartic acid − and combinations of these − were found to be the most effective acids. An azacrown with two attached imidazoles led to more pronounced effects, resulting, in the presence of dicarboxylic acids, in increases of up to k rel = 5 in comparison with the effect of the metal alone. Incorporation of imidazole and carboxylic group within a single synthetic cofactor resulted in the largest Eu III catalysis rate increase so far observed with plasmid DNA.