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Effects of coenzyme Q10 treatment on antioxidant pathways in normal and streptozotocin‐induced diabetic rats
Author(s) -
Rauscher Frederick M.,
Sanders Ruth A.,
Watkins John B.
Publication year - 2001
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/1099-0461(2001)15:1<41::aid-jbt5>3.0.co;2-z
Subject(s) - coenzyme q10 , glutathione peroxidase , glutathione reductase , lipid peroxidation , oxidative stress , chemistry , endocrinology , antioxidant , medicine , superoxide dismutase , glutathione , streptozotocin , diabetes mellitus , pharmacology , biochemistry , enzyme
Coenzyme Q 10 is an endogenous lipid soluble antioxidant. Because oxidant stress may exacerbate some complications of diabetes mellitus, this study investigated the effects of subacute treatment with exogenous coenzyme Q 10 (10 mg/kg/day, i.p. for 14 days) on tissue antioxidant defenses in 30‐day streptozotocin‐induced diabetic Sprague‐Dawley rats. Liver, kidney, brain, and heart were assayed for degree of lipid peroxidation, reduced and oxidized glutathione contents, and activities of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase. All tissues from diabetic animals exhibited increased oxidative stress and disturbances in antioxidant defense when compared with normal controls. Treatment with the lipophilic compound coenzyme Q 10 reversed diabetic effects on hepatic glutathione peroxidase activity, on renal superoxide dismutase activity, on cardiac lipid peroxidation, and on oxidized glutathione concentration in brain. However, treatment with coenzyme Q 10 also exacerbated the increase in cardiac catalase activity, which was already elevated by diabetes, further decreased hepatic glutathione reductase activity, augmented the increase in hepatic lipid peroxidation, and further increased glutathione peroxidase activity in the heart and brain of diabetic animals. Subacute dosing with coenzyme Q 10 ameliorated some of the diabetes‐induced changes in oxidative stress. However, exacerbation of several diabetes‐related effects was also observed. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:41–46, 2001