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Cytochromes P450 involved with benzene metabolism in hepatic and pulmonary microsomes
Author(s) -
Powley Mark W.,
Carlson Gary P.
Publication year - 2000
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/1099-0461(2000)14:6<303::aid-jbt2>3.0.co;2-8
Subject(s) - cyp2e1 , isozyme , cytochrome p450 , metabolism , microsome , benzene , cyp1a2 , chemistry , biochemistry , carcinogen , drug metabolism , enzyme , toxicology , biology , organic chemistry
Benzene is an occupational hazard and environmental toxicant found in cigarette smoke, gasoline, and the chemical industry. The major health concern associated with benzene exposure is leukemia. The toxic effects of benzene are dependent on its metabolism by the cytochrome P450 enzyme system. Previous research has identified CYP2E1 as the primary P450 isozyme responsible for benzene metabolism at low concentrations, whereas CYP2B1 is involved at higher concentrations. Our studies using microsomal preparations from human, mouse, and rat indicate that CYP2E1 is the P450 isozyme primarily responsible for benzene metabolism in lung and in liver. CYP2B isozymes have little involvement in benzene metabolism by either lung or liver. Our results also indicate that isozymes of the CYP2F subfamily may play a role in benzene metabolism by lung. © 2000 John Wiley & Sons, Inc. J Biochem Mol Toxicol 14:303–309, 2000