Open AccessPlasma β‐ N ‐acetylhexosaminidase isoenzyme composition and temperature conversion factors
Author(s) -
Casal J. Antonio,
Pérez Luis F.,
Tutor J. Carlos
Publication year - 2000
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/1098-2825(20001212)14:6<327::aid-jcla13>3.0.co;2-8
Subject(s) - isozyme , chromogenic , composition (language) , substrate (aquarium) , chemistry , enzyme , beta (programming language) , analytical chemistry (journal) , chromatography , biochemistry , biology , computer science , ecology , linguistics , philosophy , programming language
Using the chromogenic substrate 3,3′‐dichlorophenylsulfonphthaleinyl‐ N ‐acetyl‐β‐d‐glucosaminide for the activity determination of plasma β‐ N ‐acetylhexosaminidase (Hex), the temperature conversion factors (TCF) offer a highly significant positive correlation with the relative proportion of Hex B isoenzyme ( P < 0.001). The calculation of TCF 37°/25°C allows the isoenzyme composition of Hex to be determined quickly and cheaply. The results may be superimposed over those obtained in a previously described method based on the calculation of the enzyme’s activation energy using four temperatures. However, the use of TCF 37°/30°C does not appear to comply with the required demands. J. Clin. Lab. Anal. 14:327–329, 2000. © 2000 Wiley‐Liss, Inc.