Open AccessIdentification of genes highly expressed in G2‐arrested Chinese hamster ovary cells by differential display analysis
Author(s) -
Sasaki Yasushi,
Itoh Fumio,
Suzuki Hiromu,
Kobayashi Toshihisa,
Kakiuchi Hideki,
Hareyama Masato,
Imai Kohzoh
Publication year - 2000
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/1098-2825(20001212)14:6<314::aid-jcla11>3.0.co;2-o
Subject(s) - chinese hamster ovary cell , biology , gene , differential display , microbiology and biotechnology , cell cycle , genetics , complementary dna , cell culture
Abnormal cell cycle regulation is believed to be an important step in tumorigenesis. In mammalian cells, DNA damage commonly leads to cell cycle arrest in G2; however, little is known about the detailed biochemical mechanisms underlying the DNA damage‐induced G2 arrest. In order to identify genes differentially expressed in association with G2 arrest, differential display analysis was performed between exponentially growing Chinese hamster ovary (CHO) cells and G2‐arrested CHO cells induced by etoposide, SN‐38, or X‐radiation. We identified five cDNA clones whose expression was up‐regulated in G2‐arrested CHO cells. Sequence analysis revealed that three clones were homologous to known genes: isogene I of translation initiation factor eIF‐4A, ribosomal protein L13, and translation repressor NAT1. The remaining two clones showed no homology to known genes. These results indicate that DNA damage can alter the expression of multiple genes, including translational regulators. J. Clin. Lab. Anal. 14: 314–319, 2000. © 2000 Wiley‐Liss, Inc.