Open AccessModified salting‐out method for DNA isolation from newborn cord blood nucleated cells
Author(s) -
Noguera Nélida Inés,
Tallano Carina Elizabet,
Bragós Irma Margarita,
Milani Angela Cristina
Publication year - 2000
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/1098-2825(20001212)14:6<280::aid-jcla6>3.0.co;2-0
Subject(s) - salting out , nucleic acid , dna extraction , dna , chromatography , phenol extraction , chemistry , globin , cord blood , chloroform , microbiology and biotechnology , polymerase chain reaction , biochemistry , biology , gene , rna , genetics , organic chemistry , aqueous solution
The present work describes modification of a widely used salting‐out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β‐globin gene. The salting‐out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re‐extract the samples. J. Clin. Lab. Anal. 14:280–283, 2000. © 2000 Wiley‐Liss, Inc.