Open AccessDetection of mycobacterial DNA in papulonecrotic tuberculid lesions by polymerase chain reaction
Author(s) -
Quirós E.,
Bettinardi A.,
Quirós A.,
Piédrola G.,
Maroto M.C.
Publication year - 2000
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/1098-2825(2000)14:4<133::aid-jcla1>3.0.co;2-7
Subject(s) - polymerase chain reaction , tuberculosis , dna , southern blot , medicine , virology , biology , microbiology and biotechnology , gene , pathology , genetics
Tuberculids are a heterogenous group of cutaneous lesions. Recent discoveries of M. Tuberculosis DNA in these lesions by PCR suggest that M. tuberculosis could play a role in their pathogenesis. The aim of this study was to demonstrate the presence of M. tuberculosis DNA by polymerase chain reaction in papulonecrotic tuberculid lesions. Skin biopsy specimens from ten patients with papulonecrotic tuberculid lesions (histopathologic features) were studied. All of them tested solidly positive in a tuberculin intradermal test. A gene‐amplification PCR, using primers capable of amplifying DNA in the M. tuberculosis complex, was performed to detect M. tuberculosis DNA in the lesions. A 285‐bp sequence specific of M. tuberculosis complex was amplified and confirmed by Southern‐blot hybridation with a 32 p 5′‐labelled internal probe. No inhibitors were detected in the negative PCR samples. The PCR technique makes the detection of mycobacterial DNA in tuberculids a possibility, and therefore provides a rational basis for antituberculous therapy and for the clinical management of these disorders. J. Clin. Lab. Anal. 14:133–135, 2000. © 2000 Wiley‐Liss, Inc.