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Elevated levels of the polyadenylation factor CstF 64 enhance formation of the 1kB Testis brain RNA‐binding protein (TB‐RBP) mRNA in male germ cells
Author(s) -
Chennathukuzhi Vargheese M.,
Lefrancois Stephane,
Morales Carlos R.,
Syed Viqar,
Hecht Norman B.
Publication year - 2001
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/1098-2795(20010401)58:4<460::aid-mrd15>3.0.co;2-f
Subject(s) - polyadenylation , biology , rna binding protein , messenger rna , cleavage stimulation factor , cleavage and polyadenylation specificity factor , microbiology and biotechnology , precursor mrna , three prime untranslated region , binding protein , untranslated region , rna , rna splicing , gene , biochemistry
The single copy mouse Testis Brain RNA‐Binding Protein (TB‐RBP) gene encodes three mRNAs of 3.0, 1.7, and 1.0 kb which only differ in their 3′ UTRs. The 1 kb TB‐RBP mRNA predominates in testis, while somatic cells preferentially express the 3.0 kb TB‐RBP mRNA. Here we show that the 1 kb mRNA is translated several‐fold more efficiently than the 3 kb TB‐RBP in rabbit reticulocyte lysates and cells with elevated levels of the 1 kB TB‐RBP mRNA express high levels of TB‐RBP. To determine if the cleavage stimulatory factor CstF 64 can modulate the alternative splicing of the TB‐RBP pre‐mRNA and therefore TB‐RBP expression, CstF 64 levels and binding to alternative polyadenylation sites were examined. CstF 64 is abundant in the testis and preferentially binds to a distal site in the TB‐RBP pre‐mRNA that produces the 3 kb TB‐RBP. Moreover, upregulation or overexpression of CstF 64 increases the poly(A) site selection for the 1 kb TB‐RBP mRNA. We propose that the level of the polyadenylation factor CstF 64 modulates the level of TB‐RBP synthesis in male germ cells by an alternative processing of the TB‐RBP pre‐mRNA. Mol. Reprod. Dev. 58:460–469, 2001. © 2001 Wiley‐Liss, Inc.