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Effect of hypoxia on DNA fragmentation in different brain regions of the newborn piglet
Author(s) -
David JeanClaude,
Grongnet JeanFrançois
Publication year - 2000
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/1098-2795(200010)57:2<153::aid-mrd6>3.0.co;2-7
Subject(s) - biology , hypoxia (environmental) , cerebellum , striatum , fragmentation (computing) , dna fragmentation , hippocampus , apnea , hypothalamus , endocrinology , medicine , oxygen , biochemistry , chemistry , programmed cell death , ecology , apoptosis , organic chemistry , dopamine
DNA fragmentation has been studied in different regions of the newborn piglet brain following different times of normobaric hypoxia (5% O 2 , 95% N 2 ). After 1 hr of hypoxia, fragmented DNA was observed in cerebellum, cortex, hippocampus, and striatum but not in hypothalamus. More fragmentation occurred in these areas of the brain when the animals were kept under hypoxia for times up to 8 hr 45 min. When the animals were submitted to hypoxia for two and a half hours, integrity of DNA was recovered respectively after 3 hr of exposure to the ambient atmosphere in hippocampus and striatum, but 4 hr of recovery were necessary for cerebellum and cortex. These results are discussed in terms of the consequences of neonatal hypoxia and apnea for newborn infants and economical impact for farm animals. Mol. Reprod. Dev. 57:153–158, 2000. © 2000 Wiley‐Liss, Inc.