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Expression, immunolocalization and sperm‐association of a protein derived from 24p3 gene in mouse epididymis
Author(s) -
Chu SinTak,
Lee YingChu,
Nein KuangMing,
Chen YeeHsiung
Publication year - 2000
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/1098-2795(200009)57:1<26::aid-mrd5>3.0.co;2-4
Subject(s) - biology , epididymis , gene expression , complementary dna , microbiology and biotechnology , gene , sperm , biochemistry , genetics
The cDNA sequence for 24p3 protein in ICR mouse epididymal tissue was determined by PCR using primers designed according to the cDNA sequence derived from 24p3 protein in mouse uterine tissue. In the present study, 24p3 protein was immunolocalized in the epithelial cells and lumen of mouse epididymis. Both immunoblot analysis for protein and northern blot analysis for mRNA level showed a declining gradient of 24p3 expression from the caput to caudal region of the epididymis. The 24p3 protein was undetectable in the testis. These findings suggest that the 24p3 protein is a caput‐initiated secretory protein in the mouse epididymis. A postnatal study revealed that 24p3 gene expression occurred in mice at the age of 14 days, before the completion of epididymal differentiation. This expression remained at a constant level until epididymal differentiation was completed. We also found that the secreted 24p3 protein interacted predominantly with the acrosome of caudal spermatozoa. Our findings suggest that the epididymal 24p3 protein is a caput‐initiated and sperm‐associated gene product and may be important in the reproductive system. Mol. Reprod. Dev. 57:26–36, 2000. © 2000 Wiley‐Liss, Inc.