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Effects of platelet activating factor on capacitation and acrosome reaction in mouse spermatozoa
Author(s) -
Huo and LiJun,
Yang ZengMing
Publication year - 2000
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/1098-2795(200007)56:3<436::aid-mrd14>3.0.co;2-l
Subject(s) - capacitation , acrosome reaction , platelet activating factor , acrosome , biology , andrology , ionophore , incubation , sperm , medicine , endocrinology , biochemistry , membrane
Platelet activating factor (PAF) plays an important role in mammalian reproduction. The aim of this study was to investigate the effects of PAF on capacitation and acrosome reaction of mouse spermatozoa by chlortetracycline (CTC) fluorescence assay and coomassie blue staining. The percentage of capacitated mouse spermatozoa was increased ( P < 0.05) by incubation with 50 ng/ml PAF for 20–120 min. The peak response occurred between 80 to 100 min of exposure to PAF. In contrast, the effects of PAF on acrosome reaction may be not receptor‐mediated since lyso‐PAF had the same effects. Ionophore A23187 stimulated an increase in acrosome‐reacted spermatozoa of PAF‐treated spermatozoa, but not of lyso‐PAF‐treated ones. These results suggest that PAF mainly acts on sperm capacitation. Mol. Reprod. Dev. 56:436–440, 2000. © 2000 Wiley‐Liss, Inc.