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Microvascular repair with 1‐mm polytetrafluoroethylene (PTFE) grafts: Effect of recombinant tissue‐type plasminogen activator (rt‐PA) on the patency rate and healing process
Author(s) -
Lykoudis Efstathios G.,
Papalois Apostolos E.,
Gravvanis Andreas I.,
Frangia Konstantina B.,
Stamatopoulos Constantinos N.,
Ioannovich John D.
Publication year - 2000
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/1098-2752(2000)20:7<324::aid-micr3>3.0.co;2-q
Subject(s) - medicine , polytetrafluoroethylene , plasminogen activator , t plasminogen activator , fibril , collagen fibril , saline , surgery , urology , anatomy , chemistry , biochemistry , organic chemistry
The present study assesses the effect of recombinant tissue‐type plasminogen activator (rt‐PA) on the patency rate and healing process of microvascular polytetrafluoroethylene (PTFE) grafts. Wistar rats were used, divided into four groups of 25 animals each. After dissection of the carotid artery a segment of the vessel, 1 cm long, was resected and replaced by equal length graft. Two different type fibril length (30‐ or 60‐μm) grafts of the same wall thickness (0.18 mm) were used. Normal saline or 3 mg/kg of body weight of rt‐PA was applied locally in each group of different fibril length grafts. Patency tests were performed at 15 min and 4 weeks after blood flow was reestablished. All grafts were harvested and examined histologically. The results showed that local application of rt‐PA improves patency statistically significantly in both types of fibril length grafts. Patency in 60‐μm fibril length grafts was statistically significantly higher than that of 30‐μm fibril length grafts, whether rt‐PA was used or not. The use of rt‐PA had no influence on the healing process of either type of graft. © 2000 Wiley‐Liss, Inc. MICROSURGERY 20:324–330 2000