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New multiparametric monitoring approach for real‐time evaluation of drug tissue interaction in vivo
Author(s) -
Mayevsky Avraham,
Rogatsky Gennady G.,
Sonn Judith
Publication year - 2000
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/1098-2299(200007/08)50:3/4<457::aid-ddr29>3.0.co;2-p
Subject(s) - in vivo , drug , pharmacology , biomedical engineering , drug action , chemistry , pathology , medicine , biology , microbiology and biotechnology
Drug–tissue interactions can be evaluated at various levels of biological organization. The effects of different drugs are widely tested in various sliced tissues or intact organs under in vitro conditions. Very few techniques have been applied to monitor functional state of organs under in vivo conditions. In this overview, a new multiparametric monitoring approach (MPA) is presented that could be applied to various organs (brain, heart, kidney) in vivo exposed to compound effects. Tissue blood flow is evaluated by laser Doppler flowmetry, mitochondrial function by surface fluorometry reflectometry of NADH, and membrane integrity by extracellular level of K + , Ca 2+ , H + , or Na + . Other parameters, e.g., EEG, ECG, ICP, or temperature represent the functional state of the organ. The construction of the multiprobe assembly was adopted to various experimental as well as clinical situations to monitor various organs. Typical examples of the application of the MPA to test the effects of alcohol on the rat brain or antiischemic compounds in the gerbil brain are shown. The results collected using the MPA methodology allowed the differentiation between the various effects of a tested compound at the biochemical and physiological levels and provided suggestions as to a possible mode of action under in vivo conditions. Drug Dev. Res. 50:457–470, 2000. © 2000 Wiley‐Liss, Inc.