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An identical HMGIC‐LPP fusion transcript is consistently expressed in pulmonary chondroid hamartomas with t(3;12)(q27–28;q14–15)
Author(s) -
Rogalla Piere,
Lemke Inga,
Kazmierczak Bernd,
Bullerdiek Jörn
Publication year - 2000
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/1098-2264(2000)9999:9999<1::aid-gcc1043>3.0.co;2-n
Subject(s) - biology , exon , fusion gene , fusion , genetics , gene , microbiology and biotechnology , linguistics , philosophy
The high frequency of the t(3;12)(q27–28;q14–15) in lipomas and pulmonary chondroid hamartomas (PCHs) makes the HMGIC‐LPP fusion gene the most common fusion gene in a human tumor known so far. Nevertheless, there is no in‐depth molecular analysis of the HMGIC‐LPP fusion transcripts in PCHs. Certainly, a possible molecular variability of the HMGIC‐LPP fusion may contribute to a better understanding of the histologic differences between lipomas and PCHs and the intratumoral histologic heterogeneity of PCHs. By RT‐PCR and restriction analysis, we have investigated the HMGIC‐LPP fusion transcripts in a series of 13 PCHs with t(3;12)(q27–28;q14–q15). HMGIC‐LPP fusion transcripts of identical size were found in all PCHs tested. In all tumors investigated, the fusion transcripts had the same structure, i.e., exons 1 to 3 of HMGIC and exons 9 to 11 of LPP encoding a protein composed of three AT‐hooks and two LIM‐domains. Our results clearly show that neither the histologic differences between lipomas and PCHs nor the histologic heterogeneity of PCHs can be explained by a molecular diversity of the HMGIC‐LPP fusion transcript. © 2000 Wiley‐Liss, Inc.