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Chromosome 18 suppresses the tumorigenicity of prostate cancer cells
Author(s) -
Padalecki Susan S.,
JohnsonPais Teresa L.,
Killary Ann M.,
Leach Robin J.
Publication year - 2001
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1093>3.0.co;2-l
Subject(s) - du145 , biology , chromosome , prostate cancer , cancer research , cancer , chromoplexy , population , genetics , suppressor , gene , microbiology and biotechnology , lncap , medicine , pca3 , environmental health
Microcell‐mediated chromosome transfer allows for the introduction of normal chromosomes into tumor cells in an effort to identify putative tumor suppressor genes. We have used this approach to introduce an intact copy of chromosome 18 into the prostate cancer cell line DU145, and independently to introduce human chromosomes 8 and 18 into the prostate cancer cell line TSU‐PR1. Introduction of an extra copy of human chromosome 8 had no effect on the growth properties in vitro or the tumorigenicity in vivo of TSU‐PR1 cells. However, microcell hybrids containing an introduced copy of human chromosome 18 exhibited a longer population doubling time, retarded growth in soft agar, and slowed tumor growth in athymic nude mice. These experiments provide functional evidence for the presence of one or more tumor suppressor genes on human chromosome 18 that are involved in prostate cancer. © 2001 Wiley‐Liss, Inc.