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Analysis of genetic alterations in primary nasopharyngeal carcinoma by comparative genomic hybridization
Author(s) -
Fang Yan,
Guan XinYuan,
Guo Yin,
Sham Jonathan S.T.,
Deng Manquan,
Liang Qiwan,
Li Huimei,
Zhang Hongen,
Zhou Hang,
Trent Jeffrey
Publication year - 2001
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1086>3.0.co;2-d
Subject(s) - loss of heterozygosity , comparative genomic hybridization , biology , chromosome , nasopharyngeal carcinoma , chromosomal region , genetics , cytogenetics , chromosomal fragile site , chromosome 3 , genome , cancer research , gene , medicine , radiation therapy , allele
To identify genetic alterations associated with the development and progression of human nasopharyngeal carcinoma (NPC), 57 tumors were analyzed by comparative genomic hybridization (CGH). In 47 cases, chromosomal imbalances were found. Several recurrent chromosomal abnormalities were identified in the present study. The most frequently detected chromosomal gains involved chromosome arms 12q (24 cases, 51%), 4q (17 cases, 36%), 3q (16 cases, 34%), 1q (15 cases, 32%), and 18q (15 cases, 32%). Common regions of gain involved 12q13–q15, 4q12–q21, and 3q21–q26. High‐copy‐number increases of chromosomal materials were detected in four chromosomal regions, 3q21–q26.2, 4p12–q21, 8p, and 12q14–q15. The most frequently detected loss of chromosomal materials involved chromosome arms 16q (26 cases, 55%), 14q (21 cases, 45%), 1p (20 cases, 43%), 3p (20 cases, 43%), 16p (19 cases, 40%), 11q (17 cases, 36%), and 19p (16 cases, 34%). The most common regions of loss involved 14q24–qter, 1pter–p36.1, 3p22–p21.3, 11q21–qter, and the distal region of 19p. Genomic alterations detected by CGH were compared and found to be largely consistent with those identified in banding analysis and loss of heterozygosity studies. However, several previously unrecognized recurrent alterations were also identified in the present study, including gain of 4q and 18q, and loss of 16q, 14q, and 19p. In addition, gain of 1q, 8q, 18q, and loss of 9q showed a statistically significant association with advanced clinical stages ( P < 0.05). Identification of recurrent sites of chromosomal gain and loss identify regions of the genome that may contain oncogenes or tumor suppressor genes, respectively, which may be involved in the tumorigenesis of NPC. Published 2000 Wiley‐Liss, Inc.