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Multicolor fluorescence in situ hybridization with peptide nucleic acid probes for enumeration of specific chromosomes in human cells
Author(s) -
Taneja Krishan L.,
Chavez Elizabeth A.,
Coull James,
Lansdorp Peter M.
Publication year - 2001
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1054>3.0.co;2-m
Subject(s) - peptide nucleic acid , nucleic acid , metaphase , interphase , microbiology and biotechnology , fluorescence in situ hybridization , biology , dna , hybridization probe , chromosome , nucleic acid thermodynamics , in situ hybridization , fish <actinopterygii> , in situ , computational biology , genetics , chemistry , gene , base sequence , messenger rna , organic chemistry , fishery
In previous studies, we showed that peptide nucleic acid (PNA) probes have significant advantages over conventional synthetic RNA or DNA probes in FISH procedures for detecting telomeric and trinucleotide repeat sequences. Here, we report that directly labeled PNA probes recognizing chromosome‐specific repeat sequences are also powerful tools for detecting and enumerating specific chromosomes in interphase and metaphase cells. This is illustrated by multicolor FISH experiments with cells from normal individuals and patients with numerical sex chromosome aberrations. © 2000 Wiley‐Liss, Inc.