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Interstitial deletion of 11q13 sequences in HeLa cells
Author(s) -
Srivatsan Eri S.,
Bengtsson Ulla,
Manickam Pachiappan,
Benyamini Payam,
Chandrasekharappa Settara C.,
Sun Chi,
Stanbridge Eric J.,
Redpath J. Leslie
Publication year - 2000
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1024>3.0.co;2-p
Subject(s) - biology , microbiology and biotechnology , hela , tumor suppressor gene , southern blot , men1 , gene , genetics , carcinogenesis , cell , multiple endocrine neoplasia
Previous cytogenetic and molecular genetic studies have shown that the HeLa (cervical carcinoma) cell line D98/AH‐2 contains two apparently normal copies of chromosome 11 and additional 11q13–25 material translocated onto a chromosome 3 marker. To determine the 11q13 breakpoint, we performed fluorescence in situ hybridization (FISH) using 18 different 11q13 specific BAC (bacterial artificial chromosome) and cosmid probes spanning a 5.6 Mb interval. Markers localized to the multiple endocrine neoplasia type 1 ( MEN 1) gene (menin) were also included in the analysis. The FISH study identified an interstitial deletion between markers D11S449 and GSTP1, an interval of 2.3 Mb, in the marker chromosome. This deletion did not include the MEN 1 gene. Because point mutations and methylations can inactivate the MEN 1 gene, single stranded conformational polymorphism (SSCP) and Northern and Western blot analyses were performed with MEN 1 specific probes and antibody. SSCP did not reveal mutations of the MEN 1 gene in HeLa or in seven other cervical cancer cell lines. Northern and Western blot studies revealed normal levels of expression of this gene in the cervical cancer cell lines as well as in HeLa cell derived tumorigenic hybrids. Because deletions of tumor suppressor genes often occur in cancer progression, we hypothesize that the inactivation of a tumor suppressor gene other than MEN 1, localized to the 2.3 Mb interval on 11q13, might play a role in the abnormal growth behavior of HeLa cells in vitro or in vivo. © 2000 Wiley‐Liss, Inc.