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Peripheral blood progenitor cell mobilization in three groups of subjects: A comparison of leukapheresis yield and timing
Author(s) -
Linn Y.C.,
Heng K.K.,
Rohimah S.,
Goh Y.T.
Publication year - 2000
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/1098-1101(2000)15:4<217::aid-jca1>3.0.co;2-z
Subject(s) - leukapheresis , medicine , apheresis , cd34 , progenitor cell , peripheral blood , mobilization , chemotherapy , yield (engineering) , surgery , immunology , urology , stem cell , platelet , history , genetics , materials science , archaeology , metallurgy , biology
In this review, we analyse the peripheral blood progenitor cell mobilization yield of three categories of subjects including group 1, healthy allogeneic donors given growth factors; group 2, patients with haematological malignancies mobilized with chemotherapy followed by growth factors; and group 3, patients with solid tumours mobilized with growth factors alone. A wide variation amongst subjects of the same category was observed. Group 1 and group 2 patients mobilized to a similar degree with a mean CD34 + yield/kg of 3.44 × 10 6 and 3.39 × 10 6 respectively, for a standardized 2.5 times blood volumes processed. This is superior to group 3 patients mobilized with growth factors alone who yielded only 0.99 × 10 6 /kg. A good correlation between peripheral blood CD34 + count and leukapheresis yield was observed for all three groups. For healthy donors, prescheduled leukapheresis on day 5 after growth factors commencement predicts good yield, obviating the need for CD34 monitoring. On the contrary, most cancer patients mobilized with growth factors alone as in group 3 have inadequate single collection. They invariably require cumulative yield of several collections for adequate dose and hence predicting timing with peripheral blood CD34 + count is not useful. In group 2 patients mobilized with chemotherapy followed by growth factors, we find that a peripheral blood CD34 + count of 11/μL predicts collection of more than 1 × 10 6 CD34 + cell/kg/2.5 blood volumes, thus helping to maximize yield and resources. J. Clin. Apheresis, 15:217–223, 2000. © 2000 Wiley‐Liss, Inc.