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Fast detection of high molecular weight substances in wastewater based on an enzymatic hydrolysis combined with the Arxula BOD sensor system
Author(s) -
Tag Kristina,
Kwong Alex W K,
Lehmann Matthias,
Chan Chiyui,
Renneberg Reinhard,
Riedel Klaus,
Kunze Gotthard
Publication year - 2000
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/1097-4660(200011)75:11<1080::aid-jctb311>3.0.co;2-#
Subject(s) - starch , cellulase , cellulose , hydrolysis , biochemical oxygen demand , yeast , chromatography , chemistry , wastewater , amylase , enzymatic hydrolysis , protease , biochemistry , enzyme , chemical oxygen demand , environmental engineering , environmental science
A microbial amperometric sensor based on the yeast Arxula adeninivorans LS3 was tested for its suitability to measure the biochemical oxygen demand incurred by the use of high molecular weight substances, such as starch, cellulose and milk powder. As with other sensors, the untreated samples varied strongly from the corresponding BOD 5 . Digestion of macromolecules with α‐amylase, cellulase and protease for 30 min at 37 °C resulted in sensor BOD values which correlated better with BOD 5 values when corrected for the enzyme blank signal. © 2000 Society of Chemical Industry